The German Environment Agency has conducted tests to determine the ecotoxicity of TNT and its metabolites 2-ADNT and 4-ADNT, as well as other munition compounds (1,3-DNB and 2,6-DNT) by using two marine diatom species (Phaeodactylum tricornutum and Skeletonema marinoi) in accordance with DIN EN ISO 10253 (2016) and derived effect concentrations on this basis.
Environmental quality standards (EQS), as applied in the Water Framework Directive and Marine Strategy Framework Directive, were derived for TNT in water and biota. A proposal for monitoring the EQS was made after comparing the EQS with measured environmental concentrations of TNT.